cfx opus 384 real time pcr system (Bio-Rad)
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cfx opus 384 real time pcr system
Cfx Opus 384 Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx+opus+384+real+time+pcr+system/Hard-Shell+384-Well+PCR+Plates/bio_rxiv__64898__2026__03__16__712096-226-5-11
Average 96 stars, based on 324 article reviews
Cfx Opus 384 Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx+opus+384+real+time+pcr+system/Hard-Shell+384-Well+PCR+Plates/bio_rxiv__64898__2026__03__16__712096-226-5-11
Average 96 stars, based on 324 article reviews
cfx opus 384 real time pcr system - by Bioz Stars,
2026-09
96/100 stars
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Quantitative RT-PCR:Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes Article Snippet: .. RT-qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) in 384-well PCR plates (Bio-Rad #MSP3842) sealed with MicroAmp Optical Adhesive Film (Applied Biosystems 4311971) and using a Article Title: The Leptospira interrogans CdaA protein is a functional diadenylate cyclase. Article Snippet: .. RT-qPCR reactions were performed in triplicate using hard-shell 384-well clear/white PCR plates (Bio-Rad) in a Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes. Article Snippet: COX-2, an inducible enzyme key to production of inflammatory prostaglandins, has tumor cellintrinsic, oncogenic activity.. Previously, we reported Cys-526-nitrosylated COX-2 (SNO-COX-2) associates with breast cancer progression and poor prognostic young onset breast cancer.. Here, using a 3D culture model of early-stage human breast cancer (MCF10DCIS cells), we report SNO-COX-2, but not nonnitrosylated COX-2, closely associated with mesenchymal cell phenotypes induced by fibrillar Col1. Article Title: Phosphorylation-driven Targeted Protein Degradation of Oncogenic β-catenin Article Snippet: Then, 1 μg of RNA was transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit with random primers (Applied Biosystems, Thermo Fisher Scientific, 4368814) and diluted to 5 ng/μl in water. .. RT-qPCR was performed on a SYBR Green Assay:Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes Article Snippet: .. RT-qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) in 384-well PCR plates (Bio-Rad #MSP3842) sealed with MicroAmp Optical Adhesive Film (Applied Biosystems 4311971) and using a Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes. Article Snippet: COX-2, an inducible enzyme key to production of inflammatory prostaglandins, has tumor cellintrinsic, oncogenic activity.. Previously, we reported Cys-526-nitrosylated COX-2 (SNO-COX-2) associates with breast cancer progression and poor prognostic young onset breast cancer.. Here, using a 3D culture model of early-stage human breast cancer (MCF10DCIS cells), we report SNO-COX-2, but not nonnitrosylated COX-2, closely associated with mesenchymal cell phenotypes induced by fibrillar Col1. Article Title: RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity Article Snippet: Following TXTL, reactions were treated with 10 μl proteinase K for 15 min at room temperature and RNA was purified using a RNA Clean & Concentrator-25 kit (Zymo Research, R1017) with on-column DNase I digestion. .. RT–qPCR was performed using an iTaq Universal SYBR Green One-Step kit (Bio-Rad, 172-5150) on a Polymerase Chain Reaction:Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes Article Snippet: .. RT-qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) in 384-well PCR plates (Bio-Rad #MSP3842) sealed with MicroAmp Optical Adhesive Film (Applied Biosystems 4311971) and using a Article Title: The Leptospira interrogans CdaA protein is a functional diadenylate cyclase. Article Snippet: .. RT-qPCR reactions were performed in triplicate using hard-shell 384-well clear/white PCR plates (Bio-Rad) in a Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes. Article Snippet: COX-2, an inducible enzyme key to production of inflammatory prostaglandins, has tumor cellintrinsic, oncogenic activity.. Previously, we reported Cys-526-nitrosylated COX-2 (SNO-COX-2) associates with breast cancer progression and poor prognostic young onset breast cancer.. Here, using a 3D culture model of early-stage human breast cancer (MCF10DCIS cells), we report SNO-COX-2, but not nonnitrosylated COX-2, closely associated with mesenchymal cell phenotypes induced by fibrillar Col1. Article Title: Phosphorylation-driven Targeted Protein Degradation of Oncogenic β-catenin Article Snippet: Then, 1 μg of RNA was transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit with random primers (Applied Biosystems, Thermo Fisher Scientific, 4368814) and diluted to 5 ng/μl in water. .. RT-qPCR was performed on a Adhesive:Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes Article Snippet: .. RT-qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) in 384-well PCR plates (Bio-Rad #MSP3842) sealed with MicroAmp Optical Adhesive Film (Applied Biosystems 4311971) and using a Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes. Article Snippet: COX-2, an inducible enzyme key to production of inflammatory prostaglandins, has tumor cellintrinsic, oncogenic activity.. Previously, we reported Cys-526-nitrosylated COX-2 (SNO-COX-2) associates with breast cancer progression and poor prognostic young onset breast cancer.. Here, using a 3D culture model of early-stage human breast cancer (MCF10DCIS cells), we report SNO-COX-2, but not nonnitrosylated COX-2, closely associated with mesenchymal cell phenotypes induced by fibrillar Col1. Real-time Polymerase Chain Reaction:Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes Article Snippet: .. RT-qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) in 384-well PCR plates (Bio-Rad #MSP3842) sealed with MicroAmp Optical Adhesive Film (Applied Biosystems 4311971) and using a Article Title: The Leptospira interrogans CdaA protein is a functional diadenylate cyclase. Article Snippet: .. RT-qPCR reactions were performed in triplicate using hard-shell 384-well clear/white PCR plates (Bio-Rad) in a Article Title: Targeted Gene Modification of HMGR Enhances Biosynthesis of Terpenoid and Phenylpropanoid Volatiles in Petunia and Lettuce Article Snippet: First-strand cDNA was synthesized using total RNA, oligo(dT) primer, and reverse transcriptase ImProm-II (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. Two-step real-time qPCR was performed on a Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes. Article Snippet: COX-2, an inducible enzyme key to production of inflammatory prostaglandins, has tumor cellintrinsic, oncogenic activity.. Previously, we reported Cys-526-nitrosylated COX-2 (SNO-COX-2) associates with breast cancer progression and poor prognostic young onset breast cancer.. Here, using a 3D culture model of early-stage human breast cancer (MCF10DCIS cells), we report SNO-COX-2, but not nonnitrosylated COX-2, closely associated with mesenchymal cell phenotypes induced by fibrillar Col1. Article Title: Targeted Gene Modification of HMGR Enhances Biosynthesis of Terpenoid and Phenylpropanoid Volatiles in Petunia and Lettuce. Article Snippet: First-strand cDNA was synthesized using total RNA, oligo(dT) primer, and reverse transcriptase ImProm-II (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. Two-step real-time qPCR was performed on a Article Title: Phosphorylation-driven Targeted Protein Degradation of Oncogenic β-catenin Article Snippet: Then, 1 μg of RNA was transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit with random primers (Applied Biosystems, Thermo Fisher Scientific, 4368814) and diluted to 5 ng/μl in water. .. RT-qPCR was performed on a Article Title: RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity Article Snippet: Following TXTL, reactions were treated with 10 μl proteinase K for 15 min at room temperature and RNA was purified using a RNA Clean & Concentrator-25 kit (Zymo Research, R1017) with on-column DNase I digestion. .. RT–qPCR was performed using an iTaq Universal SYBR Green One-Step kit (Bio-Rad, 172-5150) on a Article Title: Potential toxicity of micro- and nanoplastics in primary bronchial epithelial cells of patients with chronic obstructive pulmonary disease Article Snippet: .. Total RNA from PBEC was extracted by lysis in TRIzolTM reagent (Invitrogen) and further processed into cDNA, as previously described [ ]. qPCR was performed on the Maestro Software:Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes Article Snippet: .. RT-qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) in 384-well PCR plates (Bio-Rad #MSP3842) sealed with MicroAmp Optical Adhesive Film (Applied Biosystems 4311971) and using a Article Title: S-Nitrosylated COX-2 is a microenvironment-regulated breast cancer cell biomarker of mesenchymal phenotypes. Article Snippet: COX-2, an inducible enzyme key to production of inflammatory prostaglandins, has tumor cellintrinsic, oncogenic activity.. Previously, we reported Cys-526-nitrosylated COX-2 (SNO-COX-2) associates with breast cancer progression and poor prognostic young onset breast cancer.. Here, using a 3D culture model of early-stage human breast cancer (MCF10DCIS cells), we report SNO-COX-2, but not nonnitrosylated COX-2, closely associated with mesenchymal cell phenotypes induced by fibrillar Col1. Lysis:Article Title: Potential toxicity of micro- and nanoplastics in primary bronchial epithelial cells of patients with chronic obstructive pulmonary disease Article Snippet: .. Total RNA from PBEC was extracted by lysis in TRIzolTM reagent (Invitrogen) and further processed into cDNA, as previously described [ ]. qPCR was performed on the |